numbers 1,2 and 3 please. DNA replication is semiconservative, what does this co

Question

numbers 1,2 and 3 please.

DNA replication is semiconservative, what does this concept mean. Give brief definition. PCR is a common technique used in biochemical labs, to the right is a illustrating each essential step. Provide the name and required temperature of each step next to its arrow. Current DNA sequencing requires PCR utilizing the Sanger method, which incorporates fluorescently-labeled ddNTPs. Addition of a ddNTP terminates the extension process at that given base. Provide all possible DNA segments from the template sequence, using all four dNTPs, and ONLY ddGTP and ddATP.

Explanation / Answer

1. Semiconservative DNA replication means that the DNA replication process results in the synthesis of 2 DNA copies and each copy of DNA contains one original parent DNA strand and one newly synthesized daughter strand of DNA.

2. The steps in PCR include:Denaturation:

a. In this step the double stranded DNA is heated to 94-950C temperature in order to unwind the DNA.

b. Annealing: In this step, the temperature is lowered to 50-560C for enabling the DNA primers to attach to the template DNA.

c. Extension: In this step, the temperature is raised to 720C for Taq polymerase enzyme to act and synthesize new daughter DNA strand.

3. Template DNA strand used in sequencing is 3’ – GAACCTAGGTAC

Possible DNA segments with ddGTP

Possible DNA segments with ddATP

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