Transcribe the template. Be sure to transcribe it in the proper orientation. Ass

Question

Transcribe the template. Be sure to transcribe it in the proper orientation. Assume the appropriate promoter sequence is present for RNA polymerase recognition. Make sure you note the 5' and 3' polarity of your transcription product. Translate the transcription product into a polypeptide sequence EXACTLY as it would be done in the ribosome. Assume the following: 1) the Shine-Dalgarno sequence is present, 2) the active ribosome complex forms properly, 3) all the factors required for proper translation are available, and 4) the organism uses the normal codon-anticodon pairing that is represented in the codon table in your notes/text. If translation would not occur, explain why this does not happen. If translation should continue past the codon sequences provided (in other words, there is no signal to terminate), make indicate this in your answer. Use the codon table from your book or class notes.

Explanation / Answer

1) Transcription is the process of making mRNA from DNA with the help of RNA polymerase.

Here, the template DNA strand is 5' - TGG ATG ACG CGC CCC CAG TGA GGG - 3'

Transcribed mRNA is 3' - ACC UAC UGC GCG GGG GUC ACU CCC - 5'

Promotor sequences are located in the DNA strand about 25 basepairs upstreamor at the 5' end. Most of the eukaryotes have promotor sequence TATA. Here the promotor sequence is representes as follows.

5' ATT ATA TGG ATG ACG CGC CCC CAG TGA GGG - 3'

RNA polymerase binds to this promotor sequence and initiates transcription.

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