BIO-L111 intro to the cell, please answer all the questions on the picture I pos
ID: 91777 • Letter: B
Question
BIO-L111 intro to the cell, please answer all the questions on the picture I posted. Zoom in to read the questions clearly.Week 7 Questions Post-Lab Name 1) In today'slah you loaded 3 samples of protein onto the gel, the plasma sample, the flow through fraction and your purified antibody. For each of these samples predict whether you expect it to contain the antibody proteins, and whether you expect it to contain other cellular proteins. a. The plasma sample b. The flow through fraction c. Purified antibody 2). In your own words explain why proteins move through the sDs-PAGE gel when they are exposed to an electrical charge. 3, which protein would you expect to find closer to the wells where the sample was loaded a protein with a molecular weight of 10.000 Da or a TS.000 Da prote n? Explain your answer.
Explanation / Answer
Answer 1) a) Plasma sample will contain both antibody and cellular proteins.
b) The flow through fraction will contain only antibody proteins as the cellular proteins get separated.
c) Purified antibody contains antibody proteins only.
Answer 2) The principle of electrophoresis is based on the property of charged molecules to migrate within an electric field. The SDS (sodium dodecyl sulfate) is an anionic detergent. It denatures the protein molecules and shields the individual charge of the proteins and make them negatively charged. These negatively charged proteins are loaded into the gel towards the negative electrode. You must be knowing that opposite charges attract each other. When an electric current is applied, the negatively charged proteins attracted towards the positive electrode and hence moves along the electric current.
Answer 3) A protein with a molecular weight of 75,000 Da would be closer to the wells.
In SDS-PAGE, the proteins are separated on the basis of their size i.e molecular weight. The smaller is the size of the protein, higher is the rate of migration through the gel matrix because they encounter less resistance from the polyacrylamide mesh.
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