A biology 207 student sub cloned the promoterless, GFP gene from pcamR-GFP into

Question

A biology 207 student sub cloned the promoterless, GFP gene from pcamR-GFP into puc18-207 using a double digest. They used competent, prototrophic DH5aE. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem. Pfl 108 1715 Bsa I 1766 Sal I 2177 Amp Xmn I 2294 AIWNI 1217 ORI pUC18-207 ~2700 bp Ssp I 2501 Afl III 806 lacz promoter Sap 683 Nde 183 MCS 454 396 Hind III EcoRI Kpn I Bam HI Xba I Sal I Pst I Sac I Not I

Explanation / Answer

The total length of pCamR-GFP is 8000 bp.

GFP extends from 1995 bp to 6011 bp.

Therefore, the length of GFP is (6011 - 1995) = 4016 bp

Therefore the length of backbone of pCamR-GFP without GFP is

= 8000 - 4016

= 3984 bp

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.