You want to add D-methionine to liquid cultures of E. coli that you have grown o

ID: 914139 • Letter: Y

Question

You want to add D-methionine to liquid cultures of E. coli that you have grown overnight to see if this unusual amino acid affects the growth rate of the bacteria. To test this, you must make a 1:50 dilution of your overnight E. coli culture in fresh liquid bacterial culture media and then add the amino acid at a final concentration of 10 mM. Using a stock of D-methionine at 100 mM, how many micro-liters of this stock, how many ml of liquid bacterial culture media, and how many micro-liters of your overnight E. coli culture would you need for a final volume of 4 ml?

Explanation / Answer

Step 1: for 10-fold dilution of the methionine, we need 0.4 mL (400 uL) of the methionine stock solution.
Step 2: for 50-fold dilution of the overnight culture, the required quantity is 4/50 = 0.08 mL E. coli (80 uL)

Step 3: Culture medium required quantity = 4ml-(.4+.08)ml = 3.56 ml

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You want to add D-methionine to liquid cultures of E. coli that you have grown o

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