I am currently working on a Breast Cancer Model research as my undergraduate res

Question

I am currently working on a Breast Cancer Model research as my undergraduate research,

I will need to prepare a solution of TPA( 12-O-tetradecanoylphorbol-13-acetate) dissolved in Acetone to promote the tumor growth after the intiation stage. I am planning to make about 10 mls of the TPA solution to inject the rats twice a week for few weeks. If each rat should be treated with 10 g of TPA in 200 l of acetone which equals 0.2 mls, what are the quantities needed to prepare the solution if I want to make 10 mls of the solution with the above concentrations?

Explanation / Answer

SOLUTION:

CONCEPT: Determine the concentration of solution which you are giving to rates in terms of g per mL

Remember the conversion of units which is important in your case.

1000L = 1mL

100L = 0.1mL

200L = 0.2mL

Each rat should be treated with ( Or You have dissolved) 10g of TPA in 200L or 0.2mL of acetone

It means 0.2mL of solution contain 10g of TPA

one mL of solution will contain 10 / 0.2 = 50g

Above result is important. It means 1mL of your solution contains 50g of TPA ( This is actually concentration which you are giving to rates when you dissolve 10g in 200L of acetone)

Therefore if you want to give 10mL of solution to rates the amount of TPA = No. of mL X amount in one mL

= 10 X 50 = 500g

Therefore dissolve 500g in 10ml Or 10,000L of acetone and adminster it to rates.

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