1. The first step in this lab involved gross observation of several characterist

Question

1. The first step in this lab involved gross observation of several characteristics. After that, you followed it with the Enterotube test. Many times, between these two steps, a staining ( or series of stainings) is performed to help further narrow down the unknown bacterium. Research one of these staining procedures and describe it below.
(complete detail).

2. The Enterotube quickly and conveniently performs a series of twelve different biocheical tests. Research on of the tests and describe the procedure for performing it IF YOU ARE NOT USING AN ENTEROTUBE. ( i HAVE ASKED THIS QUESTION ONCE AND THE ENTEROTUBE WAS USED) for example use the API system.

Explanation / Answer

1. One of the staining procedures is Gram staining. The procedure for Gram staining is:

Make a smear.

heat fix it.

flood the smear with crystal violet and leave for 1 min.

Use the decolorizer like alcohol to remove the primary stain.

Counterstain of safranine is applied for about a minute.

The gram positive cells stain purple while the Gram negative cells stain red.

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2. Using the API system:

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