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need someone who understands instrumental analysis, please help with each step,

ID: 908347 • Letter: N

Question

need someone who understands instrumental analysis, please help with each step, im really confused, thank you!

4. Consider a compound that has distributed between the mobile and stationary phase within a plate in a chromatographic column. The following might represent the concentration distribution profiles in the two phases (note that the compound as depicted has a slight preference for the mobile phase).

Stationary Phase Mobile Phase

concentration is going to the left(stationary phase) concentration is going to the right(mobile phase)

a) What would happen to these concentration profiles a brief instant of time later? Explain

b) How can the phenomenon explained in 4(a) contribute to band broadening? Explain.

c) Does the contribution of this phenomenon to band broadening exhibit a dependence on flow

     rate? If so, are there any troublesome aspects to this dependence? Explain

d) What happens to this effect as the stationary phase coating is made thicker? Explain

Explanation / Answer

need someone who understands instrumental analysis, please help with each step,