Q1) Answer A, B, C, D, E, F, and G) (Total Q1): 19 marks) A map of the trpB-cont
ID: 90664 • Letter: Q
Question
Q1) Answer A, B, C, D, E, F, and G) (Total Q1): 19 marks) A map of the trpB-containing region ofthe B. subtilis chromosome is shown below. Re-draw this map in your answer booklet and use the diagram to answer your questions. All sections of this question pertain to this map. 0.3 kb trpC. 0.5 kb 0.8 kb 1.2kb 1.8 kb A) How many transcripts are made from this region of the chromosome and which genels are on the transcripts? Explain your answer with reference to features shown in the diagram. You may simply draw your answers onto the map if you (4 marks) wishExplanation / Answer
Long time duration of extension stage in PCR causes the non specific amplification. And Short interval of extension time will not sufficient to replicate the gene of interest. So generally the 1min per kb time used to amplify the gene.
As per observation there are two bands in lane-2. Lower level band is about 1.8kb (trpB).upper level band is about 2.4 kb (non specific).TrpB forward primer will bind at 3’end of the complimentary sequence. Reverse primer will bind at 5’ end of the template sequence.
In case of non specific the primer can bind at any location where it finds complimentary sequence.
This primer may be nonspecific that is amplifying the gene from different location also.
5’____________________3’ (Template sequence)
3’_____________________5’ (Complimentary sequence)
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