There are multiple questions to this post. Please answer all of them with the qu
ID: 90254 • Letter: T
Question
There are multiple questions to this post. Please answer all of them with the question number before it. Thank you in advance!
1. You are given a broth culture that is growing an unknown organism.
1a. What is the first test that you will do? Why?
The following picture is a result of the test you started in part A
1b. What is the result of this test? Would the catalase test be a helpful test for determining your unknown? What would the test help differentiate?
The following pictures are a result of the catalase test performed on your unknown
1c. What is the result of the catalase test? Based on the results of the first two tests, what is the genus of your unknown organism?
The following pictures are growth on sheep blood agar from the unknown organism
1d. Based on all of the results from the unknown organism, what is the genus and species of your unknown? Explain. Differentiate the types of hemolysis that can occur on sheep blood agar and give an example of an organism for each type of hemolysis.
Part II: You are given a broth culture with an unknown organism.
The following picture is the gram stain of your unknown organism.
2a. What is the result of the gram stain? You need to determine if this organism is a streptococcus or a staphylococcus. What is one test that can quickly distinguish between these two genus of bacteria?
The following pictures are the result of the test
2b. What is the result of the test? What is the genus of your unknown organism?
2c. You think that your unknown organism does not ferment mannitol. There are two tests that you can perform to determine if your unknown organism ferments mannitol. What are the two tests?
The following picture is the result of one of the tests you identified in part C
2d. What is the result of this test? Based on all your results, what is the genus and the species of this unknown organism?
Part III: You are given some wound material from a patient with a foot wound.
The following picture is a gram stain of the organism found in the foot wound
Microbiology A Photographic Atalis for the Laboratory
3a. What is the result of the gram stain? Based on the results of the stain, there are two organisms that can cause a skin wound. What are these two organisms?
3b. Do you need to know if this organism grows anaerobically? If so, why? How would you determine if this organism grows anaerobically?
Another test that can be used to help identify this unknown organism is the catalase test. The following picture is a catalase test from the organism found in the foot wound.
Microbiology A Photographic Atalis for the Laboratory
3c. What is the result of the catalase test?
This organism grows well on sheep blood agar. The following pictures are growth on the unknown organism on sheep blood agar
Microbiology A Photographic Atalis for the Laboratory
3d. What is the result of the growth of the unknown organism on sheep blood agar? Based on your results, what is the genus and species of the unknown organism?
Part IV: Assume that you are given a broth culture with an unknown organism.
The following picture is a gram stain of the unknown organism.
4a. What is the result of the gram stain?
The following picture depicts growth of the unknown organism on sheep blood agar
4b. What is the result of the growth of the unknown organism on sheep blood agar? What are three other types of media that would help in identifying this organism? Explain.
This unknown organism was grown on MacConkey agar. The following picture is the growth of the unknown organism on MacConkey agar.
Microbiology A Photographic Atalis for the Laboratory
4c. What is the result of the growth of the unknown organism on MacConkey agar?
The oxidase test is also useful in identifying the unknown organism. The following pictures are the result of the oxidase test with the unknown organism.
Microbiology A Photographic Atalis for the Laboratory
4d. What is the result of the oxidase test? What is the genus and species of the unknown organism?
4e. What is the medical significance of the unknown organism?
Part V: Assume you are given a broth culture with an unknown organism.
The following picture is a gram stain of the unknown organism.
Microbiology A Photographic Atalis for the Laboratory
5a. What is the result of the gram stain?
The following picture is growth of the unknown organism on sheep blood agar.
Microbiology A Photographic Atalis for the Laboratory
5b. What is the result of the growth of the unknown organism on sheep blood agar?
The following pictures are growth of the unknown organism on MacConkey agar, EMB agar, and XLD agar.
Microbiology A Photographic
Atalis for the Laboratory
5c. What are the results of the growth on MacConkey agar (1), EMB agar (2), and XLD agar (3)?
The indole test is also useful in identifying the unknown organism. The following picture is the result of the indole test with the unknown organism.
5d. What is the result of the indole test for the unknown organism? What is the genus and species of the unknown organism?
5e. What would the results for all of the IMViC tests be for this organism?
Part VI
6a. An organism grows clear and colorless on MacConkey agar and red with black centers on XLD agar. What is the genus and species of this organism?
6b. What does the gram stain look like for this organism?
6c. What are the IMViC reactions for this organism?
Part VII: Assume you are given a broth culture with an unknown organism.
The following picture is a gram stain of the unknown organism.
Microbiology A Photographic Atalis for the Laboratory
7a. What is the result of the gram stain?
The oxidase test and the catalase test are also useful in identifying the unknown organism. The following pictures are the results of the oxidase test and catalase test for the unknown organism.
Oxidase Test, Catalas
7b. What is the result of the oxidase test and the result of the catalase test?
Carbohydrate fermentation can also help identify this unknown organism. The following pictures are results from maltase, lactose, and glucose fermentation tests
Microbiology A Photographic
Atlas for the Laboratory
7c. What is the result of the maltose fermentation test (1)? What is the result of the lactose fermentation test (2)? What is the result of the glucose fermentation test (3)?
7d. What is the genus and species of the unknown organism?
Microbiology A PhotographicAtalis for the Laboratory Microbiology A Photographic
Atalis for the Laboratory
Microbiology A Photographic Atalis for the Laboratory ?@ .
Explanation / Answer
Answering 3 of the questions based on CHEGG rules:
1a The first basic test that can be done will be gram staining to check whether the species is a gram positive or a negative one.
1b. Gram positive bacteria will pick up the crystal violet color whereas gram negative will show purple color. The result indicates that they are gram positive bacteria. Catalase test will enable us to know whether the bacteria is facultative aerobe or an anerobe.
1c. The genus seems to be Strepotcoccus as catalase test is negative
1d. The genus is Streptococcus and species is Strepotococcus pneumonia as it showed hemolysis in sheep blood agar. Three different types of hemolysis ar alpha, beta and gamma. Streptococcus showsalpha, beta and gamma hemolysis, chain of round cells, when grown on blood agar plates and observed under a microscope.
2a. One test that differentiates the two is the catalase test. Staph is catalase positive and streptococcus is catalase negative.
2b. The genus is Staphylococcus as the results are catalase positive.
2c. Mannitol salt agar (selective and differential media and upon fermentation will turn yellow) and mannitol fermentation test (low pH indicates that fermentation by the organism has taken place).
2d. Since the color of the media becomes yellow the species is Staphylococcus aureus.
3a. The result is gram positive organism as the color seen is crystal violet.The organisms amay be Staphylococcus aureus or beta hemolytic Streptococcus pyogenes.
3b. Organism can be tested whether it is able to grow anerobically using catralase test. The catalase test ought to be done to differentiate betwee Staph and Strep species as Strep is negative for catalase test.
3c. The organism is a facultative aneorobe as it is negative for catalase test.
3d. Sheep blood agar shows hemolysis and the genus/species obtained is Streptococcus pyogenes.
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