Suppose you wanted to extract all of the mRNA from a eukaryotic cell. How could

Question

Suppose you wanted to extract all of the mRNA from a eukaryotic cell. How could you isolate only mBNA a. Bind it to a primer with sequence AAAAAAAAAAAAAAAAA b. Bind it to a primer with sequence ATGATGATGATGATG c. Bind it to a primer with random DNA sequences d. Bind it to a primer with multiple RNA stop sites What is typically the termination signal for transcription a. A sequence of RNA that forms a hairpin b. A sequence of RNA that codes for stop codon c. RNA polymerase only works for a fixed length of DNA d. The binding of termination proteins to the DNA Suppose the enzymes responsible for regenerating RuBP were defective. What would be the effect on the light reactions of photosynthesis and why? a. The light reactions would stop as RuBP is part of the electron transport chain b. The light reactions would stop due to a lack of NADP+ c. The light reactions would continue as they are not connected to the Calvin cycle d. the light reaction would stop due to lack of ATP Which part of the photosynthesis can occur in the absence of light? a. generation of NADPH b. electron transport c. carbon fixation and conversion to glucose d. generation of ATP What would be the effect of loss of function of the water splitting enzyme in photosystem 1 and why? a. no photosynthesis as oxygen created from water splitting is necessary b. increase photosynthesis as water splitting is the rate limiting step. c. no change in photsynthetic activity d. no photosynthesis as electrons cannot be replaced in chlorophyll Lets assume a normal molecule of Pol Ill can copy 1,000,000 base pairs of DNA before falling off the DNA molecule. What would you expect of a mutant that can only copy 1000 bp before falling off a. Pol 1 would have to be more active to remove extra RNA primers b. The mutant can only replicate chromosomes of 1000 bp or less c. The mutant would operate faster d. The mutant would be unable to copy the lagging strand Suppose you had a drug that blocked the protons from moving through ATP synthase. What would be the effect on the pH of the Thylakoid space relative to to a cell that had not been given the drug. a. There would be no difference b. Thylakoid space would be more acidic in the drugged cell c. Thylakoid space would be less acidic in the drugged cell Which enzyme did practically every carbon molecule in biological systems have to come in contact with at some point? a. water splitting enzyme b. ATP synthase c. chlorophyl d. Rubisco In DNA are GC or AT base pairs stronger and why? a. AT as they form 2 hydrogen bonds to GC pair's three b. AT because they are able to form covalent bonds c. GC as they form 3 hydrogen bonds while AT forms 2 d. GC as guanine and cytosine are more stable molecules Why are transcription and translation not linked in eukaryotes? a. eukaryotic mRNAs must be exported from the nucleus b. eukaryotic must fold outside of the nucleus c. eukaryotic mRNAs are more difficult to translate d. eukaryotes slow the process down for greater proofreading

Explanation / Answer

Answer

1. Option a is the right answer because eukaryotic mRNA have poly A tail which can be easily purified by affinity column having poly T sequence ( TTTTTTTTTT).

2. option a is the right answer because the hairpin structure in RNA breaks the interaction of RNA pol and DNA template.

3. Option c is the right answer

4. option c is the right answer

5. option d is the right answer

7. option b is the right answer because in drugged cell proton will not be able to come out from thylakoid space to stromal space, therefore, thylakoid space will be more acidic.

8. option d is the right answer

9. option c is the right answer

10. option a is the right answer

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