Suppose you want to identify the bacterial species present in a polluted stream

Question

Suppose you want to identify the bacterial species present in a polluted stream and in a clean stream. Scientists have traditionally identified bacterial species by comparing the physical and biochemical properties of laboratory-grown cultures. However, you cannot grow many of these bacteria in culture How can you identify the species from the two streams without culturing the bacteria in the laboratory? O each stream. Delect bacterial species by sequencing DNA isolated from water samples collected at o Measure the pH and chemical attributes of each stream to hypothesize what types of bacteria can exist in these environments. Find organisms in the stream that the bacteria can infect and use these hosts to keep the bacteria alive during laboratory analysis Transform a common laboratory strain of Escherichia coli with DNA isolated from the transformed E. coli. O unknown bacteria, then measure the physical and biochemical properties of the

Explanation / Answer

The correct option will be

Detect bacterial species by sequencing DNA isolated from water samples collected at each stream. This enables the correct identification of bacteria by comparing the phylogenetic tree generated from the multiple alignment of DNA sequences obtained from sequencing. Ribosomal RNA sequence is highly conserved in all the organisms, so this would be the powerful technique to identify correct bacteria.

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