You are working in the lab and discover a new factor (X) that appears to play so
ID: 901548 • Letter: Y
Question
You are working in the lab and discover a new factor (X) that appears to play some important role in wound healing. You are provided with the information that each receptor only binds a single X. In order to better characterize the interaction of X with a putative receptor, you obtain dose- response and binding data on X and obtain the following results:
{[X] } {Bound X (on 10^7 cells)} {Relative rate of proliferation}
0 0 1
10^-12M 0.2 pmols 1.7
10^-11M 1.8 pmols 4.2
10^-10M 6.0 pmols 5.2
10^-9M 10 pmols 5.4
10^-8M 12 pmols 5.4
These are the data you wish to report:
1. Approximate numbers of X receptor molecules per cell
2. Half-maximaldose.
3. Apparent Kd for the X receptor.
4. Any other details that you think might be important.
Write a very concise description containing ALL the information asked and a short paragraph describing why do you think these findings might be interesting.
Explanation / Answer
Construct Lineweaver- Burkplot plot of 1/k vs 1/[x], we get the the slope and intercept
slope = 0.188 and intercept = 4x10-12
2) Half maximal dose = Vmax/2 = 5.312/2 =2.656
3) Kd is Km = 3.99 x 10-10
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