You measure the initial rate of an enzyme reaction as a function of substrate co

Question

You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data was obtained: Create a Michaelis-Menten plot (inhibited and uninhibited should be on the same plot! You MUST use Excel and follow the provided instructions "Non-Linear Regression Fitting of Kinetics Data". Calculate the V_max in the presence and absence of the inhibitor. Calculate the K_m in the presence and absence of the inhibitor. What type of inhibitor is this? How do you know (clearly explain)?

Explanation / Answer

a) plot constructed 1/v vs.1/k. slope =Km/v and intercept=1/v

b) Vmax in the absence of inhibitor is 100 and in the presence of inhibitor is 99

c) Km in the absence of inhibitor is 0.0002 and in the presence of inhibitor is .0000495

d) It is comepetitative inhibitor since it changes Km but not much on Vmax

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