how do I find micromoles per minute using this data? Chegg Tuto 4. Determine the

Question

how do I find micromoles per minute using this data?

Chegg Tuto 4. Determine the rate of the reactions by graphing absorbencies versus time for each substrate concentration. (Put all this data on one graph in Excel. Title it appropriately.) Attach the graph to this write up. Report the rates below. Substrate volume (mL) mM NADAAw/min umol/min 5. Make a Michaelis-Menten graph. Plot rate vs. [S] mM. Estimate Km and Vmax from the graph. Attach this graph to your write up. Km: 5. Make a Lineweaver-Burk and an Eadie-Hofstee plot. Determine Km and Vmx from each graph. Attach the graphs to your write-up. Lineweaver-Burk Eadie Hofstee Km:

Explanation / Answer

plots of absorbance versus time for each run gives a straight line. The reaction rate is related to the slope of this line, A/t, since Beer's Law relates the change in concentration to the change in absorbance.

Absorbance = molar absorptivity x path length x concentration

A = epsilon x L x c

A = Elc

sample calculation : for last reading

0.047=1/0.05* c(mol/min)/0.025

c(micromole/min)=58.75

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.