suppose you had separated the A and B chains of insulin by disulfide reduction.

Question

suppose you had separated the A and B chains of insulin by disulfide reduction. Part A. what chromatographic method should make it possible to isolate pure A and B chains? 1. ion exchange chromatography. the A chain contains no basic residues in comparison with the B chain, so ion exchange chromatography should work well. 2. Size exclusion chromatography . The difference in size between A and B are big enough for SEC to be effective. suppose you had separated the A and B chains of insulin by disulfide reduction. Part A. what chromatographic method should make it possible to isolate pure A and B chains? 1. ion exchange chromatography. the A chain contains no basic residues in comparison with the B chain, so ion exchange chromatography should work well. 2. Size exclusion chromatography . The difference in size between A and B are big enough for SEC to be effective. suppose you had separated the A and B chains of insulin by disulfide reduction. Part A. what chromatographic method should make it possible to isolate pure A and B chains? 1. ion exchange chromatography. the A chain contains no basic residues in comparison with the B chain, so ion exchange chromatography should work well. 2. Size exclusion chromatography . The difference in size between A and B are big enough for SEC to be effective.

Explanation / Answer

we can seperate A and B using ion exchange chromatography aswell as size exclusion chromatography. but in which ion exchange chromatography work well.

ion exchange chromatography. the A chain contains no basic residues in comparison with the B chain, so ion exchange chromatography should work well.

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