1. The Pribnow Box is a A. attenuator B. enhancer C. promoter D. operator 2. Whi

Question

1. The Pribnow Box is a

A. attenuator

B. enhancer

C. promoter

D. operator

2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?

A. the splicing of introns

B. addition of a poly-A at the 3' end

C. removal of introns

D. addition of a 7-methyl guanosine cap at the 5' end

3. In transcription, RNA polymerase binds at

A. a promoter

B. an operator

C. the oriC locus

D. an enhancer

4. The Central Dogma in genetics describes

A. the pattern of information flow in the cell

B. the pattern of chromosomal inheritance in populations

C. the role of mutations in disease

D. the role of promoters

5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?

A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'

B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'

C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'

D. none of the above

6. Removal of introns from RNA is by

A. primosomes

B. peptidases

C. galactosidases

D. spliceosomes

7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?

A. molecular weight

B. Svedberg units

C. map units

D. Daltons

8. During transcription, which strand is not read by the RNA polymerase?

A. coding strand

B. noncoding strand

C. template strand

D. terminator strand

9. The function of the rho protein is

A. to help terminate translation

B. to help RNA polymerase bind to the DNA

C. to help RNA polymerase find a promoter

D. to help terminate transcription

10. The unusual bases found in transfer RNAs

A. are added during transcription

B. are normal bases that have been modified after transcription

C. help the tRNAs to bind to DNA

D. ensure that the tRNA stays linear in solution

Explanation / Answer

1. answer :- The pribnow box also known as the TATA box with the consensus sequence TATAAT It is present 10 base pairs upstream of the start point of transcription .

Therefore it is a promoter .hence option c is correct.

2. Ans. In the post transcription event the slicing of RNA , capping of 7- guanasine cap at 5' end addition of poly-A-tail at the 3' end, slicing of introns do not occur because the whole mRNA molecule is to be scliced for the removal of introns.

therefore option A is correct

3. In transcription the RNApolymerase binds to the promoter which is recognised by an initiation factor sigma converting the core enzyme into a form that initiate only at the promoter .

therefore option a is correct.

4. The central dogma in genetic describe the pattern of flow of infromation in the cell from the DNA to mRNA and from mRNA to protein.

hence option a is correct.

5. The stem loop intramolecular base pairing is a pattern that occur in single stranded DNA or more commonly in RNA. This is also called as the hair pin structure , observe during termination of transcription. It is followed by a stretch of A:U base pairs.

therefore from the given option no option is correct & option d is correct .(none of the above.)

6. The removal of introns from the RNA is by spliciosome, which is a complex structure formed by the association of SnRNAs ,associated with proteins to form SnRNPs which are further associated with some accessory protein.

therefore option d is correct.

7. The rate of sedimentation of a molecule/organelle in sucrose gradient is measured in terms of Dalton

therefore option d is correct.

8.. RNA polymerase does not read the strand with polarity 5' to 3' , the strand is called as the coding strand though it does not code for anything.

therefore option a is correct.

9. The function of rho protein is to help terminate transcription (rho dependent termination)

therefore option d is correct.

10. The unusual bases found in tRNA , are the normal bases that have been modified after transcription .

the unusual base is any purine /pyrimidine ring except A,G,C,U

Therefore option b is correct.

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