1.A measurement taken, along the axis of the light source, of the amount of ligh

Question

1.A measurement taken, along the axis of the light source, of the amount of light scattered or reflected by small particles in a sample cuvette is the principle of

a. Fluorometry

b. Absorption spectrophotometry

c. Turbidimetry

d. Emission spectrophotometry

2.Wavelengths that occur in the blue-violet region have low energy compared to wavelengths composing the orange-red region.

A) True

B)   False

3.Screening tests for Porphyrins in urine, blood and feces are based on what characteristic of these compounds?

a. Color of molecules

b. Differential charge

c. Enhanced fluorescence

d. Molecular differences

Explanation / Answer

1 ) answer : c. Turbidimetry

explanation: Turbidimetry is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it. Light is passed through a filter creating a light of known wavelength which is then passed through a cuvette containing a solution. A photoelectric cell collects the light which passes through the cuvette. A measurement is then given for the amount of absorbed light

2) answer : B)   False

explanation :

wavelength : Blue -viloent < red -orange

energy :      Blue -viloent > Red -orange

3) answer : c. Enhanced fluorescence

explaantion :

Screening and quantitative tests for porphyrins in urine, blood and feces are based on the principle of enhanced fluorescence of these compounds in pH-acidic solutions

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