The homogenate must be diluted three different ways to test which dilution is wi

Question

The homogenate must be diluted three different ways to test which dilution is within the range of the protein (BGG) standard curve. You will need all three dilutions for all three tissue types, so you will end up with a total of 9 labeled microtubes. Calculate the amount of homogenized tissue and buffer needed to make 50 uL of each dilution.

I know this is simple, but what throws me off is how is a 1:1 ratio 50 of the homogenized tissue and 0 of the buffeR?

Explanation / Answer

For 1:1 dilution ratio

homogenized tissue (uL=1/2 *50ul =25ul

homogenization buffer (uL)=1/2*50ul =25ul

For 1:5 dilution ratio,

homogenized tissue (uL)=1/6*50 ul=8.33ul

homogenization buffer (uL)=5/6 *50ul=41.67 ul

for 1:10 dilution

homogenized tissue (uL)=1/11*50=4.5ul

homogenization buffer (uL)=10/11*50=45.5ul

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