4. A solution containing an unknown concentration of copper (Cu^2+) was mixed wi

Question

4. A solution containing an unknown concentration of copper (Cu^2+) was mixed with varying amounts of a standard Cu solution as described n the table below. The concentration of the standard copper solution is 400.0 mug Cu/mL. a) Make a plot (using Excel or plot paper) of the data: i.e., y-axis = Abs., x-axis = Vol. of std. Cu added (mL). b) Calculate the calibration sensitivity: c) Using the least-squares slope (m) and intercept (b) to calculate the concentration of Cu (mug/mL) in the original unknown sample. d) Calculate the value of volume of Cu added (V) when y-axis (Abs ) = 0. Use this value of V to calculate the concentration of Cu (mug/mL) in the original unknown sample.

Explanation / Answer

(b) Calibrationsensitivity:0S=mc+Sb.m=slope,c=concentrationandSbl=signalof Blank.advantage is with linear calibrationcurve,sesitivity is independent of analyte.calibration sensitivity is represented as symbol S.

(a )Relation ship between the absorbaance and concentration was directly propotional. Copper compounds The slope obtained for the graph is equivalent to the molar absorptivity.PLOT of ABSORBANCE against CONCENTRATION OF CUPER gives a LINEAR graph.

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