Lactate is quite similar in structure to beta-ketobutyrate:what effect would it
ID: 88335 • Letter: L
Question
Lactate is quite similar in structure to beta-ketobutyrate:what effect would it have had if the investigators had used lactate as the oxidizable substrate instead of beta -hydroxybutyrate? If lactate had been used instead, citrate would be formed, which would activate the TCA cycle, thereby generating additional substrates for the electron transport system (NADH and FADH_2) and rendering the experiment useless for its intended purpose. If lactate had been used instead, citrate would be formed, which would activate the TCA cycle, thereby generating additional substrates for the electron transport system (NADH and CoQ) and rendering the experiment useless for its intended purpose. If lactate had been used instead, pyruvate would be formed, which would activate the TCA cycle, thereby generating additional substrates for the electron transport system (NADH and CoQ and rendering the experiment useless for its intended purpose. If lactate had been used instead, pyruvate would be formed, which would activate the TCA cycle, thereby generating additional substrates for the electron transport system (NADH and FADH_2) and rendering the experiment useless for its intended purpose.Explanation / Answer
To determine which segments of the electron transport system (ETS) are responsible for proton pumping and hence for ATP synthesis ,investigators usually incubate isolated mitochondriainder conditions such that only a portion of the ETS is functional.one approach is to supply the mitochondria with an electron donor and an electron acceptor that are known to tap into the ETS at specific points .in addition ,inhibitors of known specificity are often added.In one such experiment ,mitochondria were incubated with beta hydroxybutyrate ,oxidized cytocrome c,ADP,Pi and cyanide .(Mitochondria have an NAD+ dependent dehydrogenase that is capable of oxidizing beta hydroxybutarate to beta ketobutyrate).
The answer is (iii)if lactate had been used instead ,pyruvate would be formed,which would activate the TCA cycle ,thereby generating additional substrate for the electron transport systen (NADH and CoQ) and rendering the experiment useless for its intended purpose .
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