Enzymes SHORT ANSWER Experiment 1: The Effect of Temperature on Catalase Activit
ID: 88150 • Letter: E
Question
Enzymes SHORT ANSWER Experiment 1: The Effect of Temperature on Catalase Activity Lab Results At which temperatures did catalase function somewhat, but not optimally? Data Analysis Why did catalase fail to function at 80 °C? Experiment 2: The Effect of Substrate Concentration on Catalase Activity Lab Results Fill in the table below with your results from the substrate concentration experiment. Substrate Concentration Results Test Tube Bubbles Formation (min) 1 2 3 4 Data Analysis Before the catalase concentration was saturated with hydrogen peroxide, why did the enzyme function vary with substrate concentration? Experiment 3: The Effect of pH on Catalase Activity Lab Results What was the pH value at which catalase functioned the best? Data Analysis Why did catalase only function in a limited range of pH values? Conclusions What are the enzyme, substrate, and product in these experiments? What role does catalase play in the mammalian liver, or any other cell it is found in?
Explanation / Answer
Answer:
Experiment 1:
a. 21.5 Celsius and 60 Celsius produced slow bubbles.
b. Enzymes need energy in order to work and while lower temperature slow down enzymes, the high temparetures can potentially alter them, or stop them from working altogether.
Experiment 2:
a. Test tube 1: Bubbles started forming right away for about 2 minutes
Test tube 2: Bubbles started forming right away for about 2 minutes
Test tube 3: Bubbles started forming right away for about 2 minutes
Test tube 4: Bubbles started forming right away for about 2 minutes
b. The enzymes had varied results before the catalyst concentration was changed due to the differences in temperature. In experiment 1, the independent variable was the temperature of each bath, and the result was the enzyme reaction or lack thereof. Whereas in experiment 2 the concentration of the substrates was the independent variable and the quantity of was bubbles the dependent variable.
Experiment 3:
a. The pH 6 buffer with 2mL of water and 8mL of 3% hydrogen peroxide functioned the best.
b. The catalyst only functioned at a limited range because a low pH, or more acidic pH, will burn the catalyst, so it will not be able to function. A higher pH, or a more basic pH, will cause the enzyme.
c. The enzyme in these experiments is the catalyst. The substrate in these experiment was the 3% hydrogen peroxide, and the product in these experiments was the creation of bubbles.
d. The role of the catalyst in the mammalian liver is too expedite the excretion of bile for the small intestine. In any cell that a catalyst is found in, its role is to speed up the whatever process is going to take place.
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