Enzymes SHORT ANSWER Experiment 1: The Effect of Temperature on Catalase Activit
ID: 88149 • Letter: E
Question
Enzymes SHORT ANSWER Experiment 1: The Effect of Temperature on Catalase Activity Lab Results At which temperatures did catalase function somewhat, but not optimally? Data Analysis Why did catalase fail to function at 80 °C? Experiment 2: The Effect of Substrate Concentration on Catalase Activity Lab Results Fill in the table below with your results from the substrate concentration experiment. Substrate Concentration Results Test Tube Bubbles Formation (min) 1 2 3 4 Data Analysis Before the catalase concentration was saturated with hydrogen peroxide, why did the enzyme function vary with substrate concentration? Experiment 3: The Effect of pH on Catalase Activity Lab Results What was the pH value at which catalase functioned the best? Data Analysis Why did catalase only function in a limited range of pH values? Conclusions What are the enzyme, substrate, and product in these experiments? What role does catalase play in the mammalian liver, or any other cell it is found in?
Explanation / Answer
ANSWER:
1) Enzymes need energy in order to work and while lower temperature slow down enzymes, the high temparetures can potentially alter them, or stop them from working altogether.
2) 21,5 Celsius and 60 Celsius produced slow bubbles.The enzymes had varied results before the catalyst concentration was changed due to the differences intemperature. In experiment 1, the independent variable was the temperature of each bath, and the result wasthe enzyme reaction or lack thereof. Whereas in experiment 2 the concentration of the substrates was theindependent variable and the quantity of was bubbles the dependent variable
3) The pH 6 buffer with 2mL of water and 8mL of 3% hydrogen peroxide functioned the best.
The catalyst only functioned at a limited range because a low pH, or more acidic pH, will burn the catalyst, soit will not be able to function. A higher pH, or a more basic pH, will cause the enzyme.
The enzyme in these experiments is the catalyst. The substrate in these experiment was the 3% hydrogenperoxide, and the product in these experiments was the creation of bubbles.
Catalase is an enzyme in the liver that breaks down harmful hydrogen peroxide into oxygen and water. When this reaction occurs, oxygen gas bubbles escape and create foam.
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