The sequence of the sense strand of a mammalian gene is: TATAATACGCGCAATACAATCTA

Question

The sequence of the sense strand of a mammalian gene is:

TATAATACGCGCAATACAATCTACAGCTTCGCGTAAATCGTAGGTAAGTTGTAATAAATATAAGTGAGTATGATACAGGCTTTGGACCGATAGATGCGACCCTGGAGGTAAGTATAGATTAATTAAGCACAGGCATGCAGGGATATCCTCCAAAAAGGTAAGTAACCTTACGGTCAATTAATTCAGGCAGTAGATGAATAAACGATATCGATCGGTTAGGTAAGTCTGAT

Determine the sequences of the mature mRNA and the encoded protein. Assume that transcription initiates at a G approximately 25 bp downstream of the TATAATA sequence, that each 5´ splice site has the sequence AG/GUAAGU, and that each 3´ splice site has the sequence CAG/G (where / marks the location of the splice).

Explanation / Answer

There are four exons (the underlined bases)
TATAATACGCGCAATACAATCTACAGCTTC(GCGTA
AATCGCAG)GTAAGTTGTAATAAATATAAGTGAGT
ATGATAGG(GCTTTGGACCGATAGATGCGACCCTG
CAG)GTAAGTATAGATTAATTAAGCACAGG(CATGCA
GGGATATCCTCCAAACAG)GTAAGTAACCTTACGG
TCAATTAATTAGG(CAGTAGATGAATAAACGATAT
CGATCGGTTACAG)TAGTCTGAT
The mature mRNA, which has a 5 cap and a 3 poly(A) tail,
therefore has the sequence
GCGUAAAUCGUAGGCUUUGGACCGAUAGAUG
CGACCCUGGAGCAUGCAGGGAUAUCCUCCAAA
UAGCAGUAGAUGAAUAAACGAUAUCGAUCGG
UUAGGU

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