Determine the consensus sequence of the -35 and -10 box of these genes. The conc

Question

Determine the consensus sequence of the -35 and -10 box of these genes. The concept of consensus sequence is explained in the text in Chapter 12 section 12.2 and figure 12.5

Suppose that there is a species of bacteria that had a promoter with a -35 box and -10 box, but the sequence of these is different from that of E. coli. The nucleotide sequences of the promoter of five different genes of this species were determined and they are shown below.

              -35 box            -10 box

geneA --------GGTAAT----------------GAACCA-------

geneB  --------GGCTCT----------------GATCTA-------

geneC  --------TGTACT----------------GATGCT-------

geneD  --------GTTACT----------------GGTCCA-------

geneE --------GGTACC----------------TATCCA-------

Explanation / Answer

Ans. The consensus sequence at -35 box region is : GGTACT

The consensus sequrnce at -10 box region is: GATCCA

A consensus sequence is a version of sequence having at each position the nucleotide most commonly found there in different example. this consensus squence is determined by aligning sequences and ascertaining the most common base found at each position in the -35 and in the -10 box.

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