DNA replication/transcription/translation question. Just the 6th question, not 7
ID: 87268 • Letter: D
Question
DNA replication/transcription/translation question. Just the 6th question, not 7th.
Given the following problems in bacterial DNA replication, state which enzyme is most likely defective. Briefly explain your reasoning. a. A replication bubble is formed, but DNA synthesis is never initiated b. stretches of RNA bases are found in the newly synthesized DNA. c. No replication bubble is formed. d. Short pieces of DNA rather than the entire replicated chromosome is discovered. Consider the following diagrams representing three different DNA molecules. If DNA polymerase III is the only enzyme present, which of these molecules can serve as substrate for DNA synthesis? For each molecule, explain why it can or cannot serve as a substrate.Explanation / Answer
Answer:
Which enzyme is most likely defective (In bacterial DNA replication):
1) A replication bubble is formed, but DNA synthesis is never initiated - DNA Polymerase III and Primase
2) Stretches of RNA base are found in the newly synthesized DNA - DNA Polymerase I (RNA removal in bacteria is performed by DNA Polymerase I using its endonuclease domain)
3) No replication bubble is formed - DNA Helicase (DNA helicases unwinds ds DNA and forms replication fork)
4) Short pieces of DNA rather than the entire replicated chromosome is discovered - DNA ligase
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.