DNA samples from four individuals were cleaved with the same restriction endonuc
ID: 853615 • Letter: D
Question
DNA samples from four individuals were cleaved with the same restriction endonuclease. The DNA fragments were separated by gel electrophoresis, transferred to a membrane, and hybridized with a DNA probe complementary to a region between sites C and D (see image below). The image of the blot (on right) shows the labeled DNA bands and molecular weight (MW) markers. The lane labels I, II, III, and IV correspond to individuals I, II, III, and IV. Assume that fragments such as CD and CE are clearly resolved in this gel system. Fragment sizes are as follows: A-B is 4 kb, B-C is 1 kb, C-D is 5 kb, and D-E is 650 bp. Individual I has five cleavage sites (A, B, C, D, and E) for the restriction endonuclease. Which individual has a point mutation(s) that eliminates ...Explanation / Answer
Restriction Site C only?
II
Restriction sites B and C?
IV
Restriction Sites A only?
There isn't enough information for that point mutation to be determined
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