1. were for an E. coli metabolic enzyme from a wild type strain and a strain wit

Question

1. were for an E. coli metabolic enzyme from a wild type strain and a strain with a double mutation, having one deletion followed by one insertion at another position. The results are shown below Amino Acid Residue 31 32 33 34 35 36 Wild Type NH PRO CYS PHE LYS THR ASN COOH Double NH HIS ALA SER ARG LEU ASN COOH Mutant a. Determine the base sequence of the mRNA for both the wild type and double mutant polypeptides. Be sure to show which bases are certain and which have uncertainty, if any b. Using your wild type mRNA sequence, show which base was deleted and the site from which it was removed. Be sure to show which bases are certain and which have uncertainty, if any c. Using your mutant sequence, show which base was added and where it was inserted. (You only need to give one solution if there are many.)

Explanation / Answer

Wild type sequence of t RNA attached amino acids-

CCC UGU UUU AAA ACU AAU

Mutant type sequence of t RNA attached amino acids-

CAU GCU UCU UUU AAA ACU AAU

Accordingly the sequence in mRNA in wild type would be-

GGG ACA AAA UUU UGA UUA

and the sequence in mutant type mRNA would be-

GUA CGA AGA GCA AAU UUA

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