DNA pol I and pol III differ in that one has 5\'-3\' polymerase activity and the
ID: 86504 • Letter: D
Question
DNA pol I and pol III differ in that one has 5'-3' polymerase activity and the other does not. one has 3'-5' exonuclease activity and the other does not. one has 5'.3' exonuclease activity and the other does not. one requires an RNA primer and the other does not. Lagging strand replication in E. coli is discontinuous because the antiparallel nature of the template DNA strands allows only one strand to be replicated continuously; lagging strand replication in the opposite direction to the unwinding of the parental duplex can occur only as the template becomes available. the replication of the same base pair takes place on the lagging strand after the replication of the complementary nucleotide on the leading strand. the processivity of DNA polymerase III is reduced when it is replicating the lagging strand. replication on the lagging strand must be reinitiated by new RNA primer synthesis at many locations. the processivity of DNA polymerase I is low, therefore, replication must begin at many origins in order to replicate the entire genome within the time available. Which of the following is least likely to be a part of an elongating replication fork complex in E. coli? Helicase/Primase Two molecules of the catalytic subunit of DNA polymerase III dnaA protein Single-stranded DNA binding protein (SSB) Sliding clamp processivity factorExplanation / Answer
ANSWER) 2. the answer will be C that is one has 5' to 3' exonuclease activiity and other doesnot have this activity.
ANSWER) 3 The answer will be A the anti-parallel nature hinders the process through which it need to very hard to replication.
ANSWER) 4 The answer will be dnaA protein which only helps to locate the origin of replication that is, it helps in locating A=T rich sites .
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