We have an protein P that binds two different sequence regions on different part

Question

We have an protein P that binds two different sequence regions on different parts of the bacterial chromosome. These two regions on the chromosome are called "operator regions", O1 and O2. For binding of P to the operator regions, the dissociation constants are 0.1 nM for 01 and 4 nM for O2. The concentration of free Pin the cell is 10 nM. What statement about the occupancy of region 01 and region 02 is TRUE? A1. Both O1 and O2 are essentially completely occupied. A2. Both O1 and O2 are partially occupied, with O2 more occupied than O1. A3. O1 is essentially completely unoccupied, but O2 binding is about 40% saturated. A4. O1 will have very high occupancy, whereas O2 is about 70% occupied. A5. Without knowing molar concentration of DNA there is not enough information to deduce occupancy.

Explanation / Answer

The answer is A4 O1 will have very high occupancy, whereas O2 is about 70% occupied. Lower the dissociation constant, higher will be the affinity.

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