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For each of the following stains, state where you think it would be mainly local

ID: 84289 • Letter: F

Question

For each of the following stains, state where you think it would be mainly localized in the cell. a. A stain that bound to G proteins b. A stain that bound to RNA and DNA polymerases c. A stain that bound to enzymes that perform primary glycosylation d. A stain that bound to enzymes that perform secondary glycosylation e. A stain that binds to hydrolytic enzymes that break down proteins and lipids f. A stain that binds to clathrin g. A stain that binds to transfer RNAs h. A stain that binds to DNA What do you think would happen to an integral membrane protein that normally is localized to the plasma membrane, and has a N-terminal signal sequence and a long hydrophobic portion near the C-terminus, under each of the following conditions? The gene that codes for the protein is mutated so it no longer has a signal sequence The gene that codes for the protein is mutated so it no longer has the long hydrophobic portion

Explanation / Answer

1 location of various enzymes inside the cell

a.G protein bound stain is seen in the cell membrane as these proteins act as molecular switches inside the cell that transmit signal into the cell from outside.

b.The stain for RNA polymerase is found in the nucleolus and of DNA polymerase is found in the nucleus.

c.Enzymes for glycosylation are found in the rough endoplasmic reticulum.

d. .Enzymes for secondary glycosylation are found in the golgi apparatus.

e.Enzymes for protein and lipid metabolism are found in the Lysosomes.

f.Clathrin stain is found on the vesicles.

g. Stain of tRNA is located on ribosome.

G.For DNA stain is seen in the nucleus.

c.

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