At a specific area of a chromosome, the sequence of nucleotides below is present

Question

At a specific area of a chromosome, the sequence of nucleotides below is present where the chain opens to form a replication fork: 3' C C T A G G C T G C A A T C C 5' An RNA is primer is formed starting at the underlined T (T) of the following represents the primer sequence? A) 5 G C C T A G G 3' B) 3' G C C T A G G 5' C) 5' A C G T T A G G 3' D) 5'.A C G U U A G G 3' E) 5' G C C U A G G 3' Use the following list of choices for the following questions: I. helicase II DNA polymerase III III. ligase IV. DNA polymerase I V. primase Which of the enzymes removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments? A) I B) II C) III D) IV E) V Which of the enzymes separates the DNA strands during replication? A) I B) II C) III D) IV E) V Which of the enzymes covalently connects segments of DNA? A) I B) II C) III D) IV E) V

Explanation / Answer

39.

D) 5’ACGUUAGG-3’

This one is the forward primer. This one is complimentary of the sequence from 3’ to 5’ prime direction of the given sequence from marked T (T)

40.

Answer-(4) DNA Polymerase-1

DNA polymerase repair the damage in DNA sequence, it also connect the Okazaki fragments followed by deletion of RNA primers and replace the DNA strand. This DNA pol 1 carries the alpha and beta segments. It works in direction of 5’à 3’which is forward reaction.

41.

Answer-(1) Helicase enzymes

Helicase enzyme binds with the nucleic acid which is essential during the process of replication. In that process it separates the two strands of DNA to be copied. It forms the replication forks over the sequence of DNA and breaks the hydrogen bond.

43.

Answer: - (3) Ligase enzyme

Ligase enzyme the ligate/join the two DNA segments using the phosphodiester bond and helps in single nucleotide break repair in the DNA sequence. The phosphodiester bond forms in between 3’ hydroxyl ends of nucleotide and 5’ phosphate of other. Because of that it is known for both function i.e. replication and repair.

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