7. Your friend (and his lab partners) are having trouble graphing their data. Al

Question

7. Your friend (and his lab partners) are having trouble graphing their data. All three partners used the same raw numbers but each made a graph that looks completely different from the graphs made by the other two. (Of course they also can’t remember which assay the numbers came from!) Study the graphs below and provide an explanation for how these three graphs could look so completely different. Be sure to state which assay the raw numbers likely came from. (10 points total)

Explanation / Answer

In absorbance graph, light absorbs according the product concentration in samples. The first graph representing wrong views because first three concentrations are showing same absorbance which is quite unfamiliar. Absorbance should increase with increasing sample and decrease with increasing solvents. Dilute sample will absorb less light than concentrated sample therefore here graph represents neither decreasing values nor increasing values.

In the second graph, each point placed in right positions. Some fluctuations are also present but unobvious characteristics are not presenting there.

In the third graph, first absorbance is very high which is impossible if we use same material as sample. That is because we use gradually decreasing or increasing concentrations of material in which first absorbance if high then other would show gradually inclination and if first absorbance gets low then it will show gradual increase.

Therefore, the second graph is representing right graph for absorbance based graph.

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