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answer only question no.3 How NADPH is produced in animal cell? What are the sou

ID: 83807 • Letter: A

Question

answer only question no.3

How NADPH is produced in animal cell? What are the sources of NADPH in Plant Cell? How many ATP molecules are generated from NADH produced during glycolysis in brain and liver? Give proper explanation. How Isozymes give advantages in metabolism? What are the pathophysiological effects of loss of function mutation of Glycogen phosphorylase and Glycogen synthase? How C-AMP regulate the activity of Glycogen phosphorylase? Pyruvate is one of the most important intermediate in the metabolism: Explain 4 fates of pyruvate in the carbohydrate metabolism. What is glyoxalate cycle? Explain why it is important for plant? Why animal does not have Glyoxalate cycle? How Dark phase of photosynthesis is regulated by light? Explain what is photorespiration? Explain CAM pathway of Photosynthesis. What is Glycogenin? What is Thermogenin? What is brown fat? Why it is called brown fat? What is the action of oligomycin?

Explanation / Answer

(A) Ethanol in formed from pyruvate in the following reaction.
Pyruvate --(1)--> Acetaldehyde --(2)--> Ethanol
(1) Pyruvate decarboxylase
(2) alcohol dehydrogenase
(B) Lactate is formed from pyruvate by the enzyme lactate dehydrogenase
Pyruvate --(1)--> Lactate
(1) Lactate dehydrogenase

  (C) Pyruvate undergoes oxidative decarboxylation to form acetyl coA
Pyruvate + NAD + CoA --(1)--> acetyl CoA, Co2 and NADH

(D) Pyruvate can be converted to oxaloacetate by pyruvate decarboxylase
(E) It can be transaminated to Alanine.

(2)

In the glyoxylate cycle,
(A) acetyl coA is converted to Citrate by citrate synthase;
(B) Citrate is converted by Isocitrate by aconitase;
(C) Isocitrate is converted to Glyoxylate by Isocitrate lyase
(D) Glyoxylate is then converted to malate by malate synthase
(E) Malate is converted to oxaloacetate by Malate dehydrogenase.

The glyoxylate cycle allows plants to grow on acetate because the cycle by-passes the decarboxylation steps of the citric acid cycle and permits the conversion of acetate into succinate-isocitrate lyase and malate synthase.

The glyoxylate cycle by-passes the carbon di oxide evolving steps of the Krebs cycle with the aid of the two enzymes isocitrate lyase and malate synthase. This is not present in animal cells.