The rll locus contains multiple. Thus, it is possible to isolate separate for th

Question

The rll locus contains multiple. Thus, it is possible to isolate separate for this locus, such as rllx and rlly. When is coinfected with rllx and rlly, a small proportion of recombinant progeny might arise if a occurs within the rll locus. These progeny could be assayed for by growth on on which only wildtype phages can grow. Although this would allow for the detection of, it would not allow for detection of the, which are to occur. Thus, the total number of recombinant phages will always be the number of the r^+ recombinants.

Explanation / Answer

The rII locus contains multiple mutational sites. Thus, it is possible to isolate separate mutants for this locus, such as rIIx and rIIy. When E.coli strain B is coinfected with rIIx and rIIy, a small proportion of recombinant progeny might arise is a crossover occurs within the rII locus. These progeny could be assayed by growth on E.coli strain K12 (lambda) on which only wildtype phages can grow. Although this would allow for the detection of r+ recombinants, it would not allow for detection of the rIIxy double mutants, which are less likely to occur. Thus, the total number of recombinant phages will always be double the number of the r+ recombinants.

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