The picture below represents an excised intron in “lariat” shape that has just b

Question

The picture below represents an excised intron in “lariat” shape that has just beenspliced out of an RNA transcript. The phosphodiester covalent bond that completes thelariat loop structure at the intron branch-point sequence “A” shown in this figureincludes:

a) the 3’-OH of the branch-point A nucleotide

b) the 5’-phosphate of the branch-point A nucleotide

c) the 2’-OH of the branch-point A nucleotide

d) the 3’-OH of the internal splice-point G nucleotide

Explanation / Answer

The introns are removed as a lariat structure in which the 5 G of the intron is joined in an unusual 2,5-phosphodiester bond to an adenosine near the 3 end of the intron

c) the 2’-OH of the branch-point A nucleotide.

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