Your lab partner wants to complete the lab exercises faster and decides to combi
ID: 82803 • Letter: Y
Question
Your lab partner wants to complete the lab exercises faster and decides to combine two of the experiments. She decides to test for the optimal pH of the experiment while varying the substrate concentration. She uses the lowest substrate concentration (0.10 mMolar) in the same tube she sets up at pH 3. The next highest substrate concentration (0.50 mMolar) was tested at a pH of 4 and so on. What are the disadvantages to her strategy? (6 points) What usable information is she likely to obtain from this approach? (4 points)
Explanation / Answer
It is stated that the researcher wants to test the optimal pH of the solution. But if she is varying the substrate concentration also then optimal pH can not be checked because we can check only one variable at a time. Other factors should be kept constant like substrate concentration, temperature etc.
Therefore she should take either of the two concentrations of the solution, either 0.10 M or 0.50M, or any concentration beetween these two.
Then she should check for the pH optimum, by checking the solution at gradients of pH. Thats how she could be sure of the optimum pH because only one variable is varying here. If two or more factors are variable, experimental error would occur.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.