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12) In eukaryotic cells, RNA Polymerase II is responsible for transcription of:

ID: 82777 • Letter: 1

Question

12) In eukaryotic cells, RNA Polymerase II is responsible for transcription of:

a) all genes

b) protein coding genes

c) most ribosomal RNA (rRNA) genes

d) transfer RNA (tRNA) genes

13) As a future biologist, you are studying alien cells from the planet Pandora in the Alpha Centauri star system. You find that these cells contain RNA consisting of 10 different nucleotide bases, and proteins consisting of 76 different amino acids. You hypothesize that the smallest possible codons in the genetic code of these alien cells would contain how many nucleotide “letters” per codon?

a) 1

b) 2

c) 3

d) 4

e) 5

14) True or False: considering what you know about the genetic code, if you know the exact sequence of amino acids in a bacterial protein, you can deduce the exact sequence of nucleotides in DNA that coded for that protein.

a) True b) False

15) How many different proteins could theoretically be produced via alternative splicing from a gene containing 3 exons and 2 introns in this order Exon-Intron-Exon-Intron- Exon? (Hint: the first Exon is always retained in the mature mRNA).

a) 2

b) 3

c) 4

d) 5

Explanation / Answer

12) RNA polymerases (RNAP) are the enzymes which produce primary transcript RNA. They are DNA dependent RNA polymerases. There are three types of RNA polymerases: I, II and III. RNA polymerase (RNAP) II is protein-protein complex found in the nucleus of eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA. RNAP II holoenzyme is recruited to the promotor regions of protein coding genes in living cells. So, the answer for this question is option (b) protein coding genes.