12) A dehydration/rehydration reaction is used to convert citrate to isocitrate

Question

12) A dehydration/rehydration reaction is used to convert citrate to isocitrate by aconitase. What co-factor is needed for this reaction?

A) NAD+

B) ATP

C) CoA

D) NADPH

E) Fe-S cluster

13) Which bases are assembled while they are attached to the ribose-phosphate sugar?

A) UMP, TMP, and CMP

B) AMP and GMP

C) ATP and GTP

D) none

E) all

14) Which enzyme converts DNA nucleotides to RNA nucleotides?

A) glutamine synthetase

B) ribonucleotide reductase (RNR)

C) ribose-2’-oxidase

D) ribose-3’-oxidase

E) none

15) Which vitamin derivative is required for purine and methionine biosynthesis?

A) Thiamine

B) Retinol

C) Biotin

D) Tetrahydrofolate

E) Ascorbic acid

16) If a bacterium that can catabolize amino acids (such as E. coli) runs out of alternative carbon sources, it will consume amino acids and the carbon backbones will go into the TCA cycle for energy. When this happens, what will happen to the pH of the medium?

A) it will stay the same

B) it will go up

C) it will go down

D) it will become hydrolyzed

E) none of the above

Explanation / Answer

12 E) Fe-S cluster is the co factor required. The rest are energy released.

13. D) none

14. E) none. ribonucleotide reductase helps in formation of dna nucleotides from rna nucleotides.

15. C) biotin

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