1. Enzyme kinetics laboratory experiment needs processing the observed spectrosc
ID: 822902 • Letter: 1
Question
1. Enzyme kinetics laboratory experiment needs processing the observed spectroscopic data to calculate the Michaelis Menten parameters for the enzyme. Two important steps in this data processing are determining Delta_t and Delta_c to compute v (Delta_c/Delta_t) for each substrate concentration. Delta_t can be obtained by selecting two time points (t1 and t2) .
Does the selection of the two time points to compute ?t have an effect on the overall outcome, e.g. would selecting the data point pair 5sec (t1), 10sec (t2) vs 20sec (t1), 25sec (t2) have an effect on the overall outcome?
2. Would the value of Delta_t have an effect on the overall outcome, e.g. would selecting Delta_t = 5sec or 10sec have an effect on the outcome? Why?
Explanation / Answer
you can select any pair of data points for time change, but condition is that you have to take the respective concentrations of that time also.
no, there will be no effect on the overall outcome because Delta_c/Delta_t will be constant at any two points
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.