actin subunit ll to maintai such a large The movement of single motor-pro he ana
ID: 82138 • Letter: A
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actin subunit ll to maintai such a large The movement of single motor-pro he analyzed directl ctly. Using polarized laser light, conewtons at dngintenceng pr in molecul cted force, r o create interfe interference patterns from zero at the center to a few that exert a centr orce, ranging from zero iphery (about 200 nm from the cen les that enter the interference pat- dual molecules that e Individual ed to the center, allowing them to be idly pushed to th ern are rapid! aptured and and moved at the ex at the experimenter's discretion. danh"optical tweezers," single kinesin molecules cro tubule that is fixed to a cover sing sitioned on a microtubule can be kinesin molecule cannot be seen ugh a single kinesin mol tically, it can be d with a silica bead and tracked indi- an be tagged with a silic by following theb owing the bead (Figure Q16- 2A). rectly b the kinesin mol esin molecule rem ains at the center of the In the absence 0 terference pattern, but in-of the microtubule. As ki ce pattern, but with ATP it moves otubule. As kinesin mov P it moves toward the plus es along the micro ule, it encounters the ch simulates the load ters the force of the interference pattern the load kinesin carries during its actual bu ad counters the effects ction in the cell. More cell. Moreover, the pressure against the silica effects of Brownian (thermal) motion, so t the position of the b on of the kinesin molecul n of the bead more accurately reflects the nesin molecule on the microtubule iti nesin molecule along otubule are shown in Figure Q16- ces 0 2B Tra icr ts lie a e oExplanation / Answer
A) The hydrolysed forms of ATP.
B) At low kinesin-to-bead ratios where the motility is due to single motors, the stoichiometry is unity.
C) 8nm
D) 8nm and kinesin molecule moves along the microtubule by hydrolysing ATP molecule.
E) Yes, step only occur if diffusion is toward the next binding site and ATP hydrolysis depict diffusive motion.
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