Please help him calculate his yield of transductants (CFU/ml). After inoculating

Question

Please help him calculate his yield of transductants (CFU/ml). After inoculating 10 ul of bacteria-phage mix from the 10^-2 diluent to the proper selective plates and incubated the plate ON at 37 degree C, there were 100 colonies grown on this plate. So, the of original transductants (CFU/ml) is___. a. 1 times 10^3 b. 1 times 10^5 d. 1 times 10^6 d. 1 times 10^6 e. 1 times 10^7 f. None of the above A growth some but the growth of some microorganisms but inhibits growth of microorganisms is a____medium. a. selective b. differential c. enriched d. none of the above A growth medium that distinguishes among different groups of bacteria based on their biological characteristics is called a____medium. a. selective b. differential c. enriched d. none of the above Media in which all components and their concentration are known are called____media a. selective b. differential c. enriched d. defined You would like to culture a bacterium that is growing in the lung of a human patient with pneumonia. Which medium would be of most use in culturing this organism? a. Mannitol salt agar b. EMB c. MacConkey's agar d. Complex medium supplemented with whole blood

Explanation / Answer

(21)d. 1 x 10^6 CFU/mL

Original CFU/ml =

Dilution factor= 1/10^2

Number of colonies= 100

Volume of sample = 0.01 mL to the plate

cfu/ml = (no. of colonies)/ (dilution factor x volume of culture plate)

=100 (1/10^2 x 0.01)

= 100 x 10^4

=1 x 10^6 CFU/mL

(22)a.Selective

(23)b.differential

(24) d.defined

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.