You have prepared a batch of competent E coli cells and found its transformation

Question

You have prepared a batch of competent E coli cells and found its transformation efficiency is 10^6 per mu g plasmid DNA. A professional scientists prepared competent E coli cells having a transformation efficiency of about 10^6 cells/mu g plasmid DNA Calculate % score of your E coli transformation efficiency (T.E). A linear fragment of DNA was studied by agarose gel electrophoresis. The picture of the gel is shown bekow. Using semi-log paper (next page) draw a stdrd curve and determine the size of DNA "A"

Explanation / Answer

Answer:

4. Transformation efficiency of your E. coli = 106

Transformation efficiency of the scientist's E. coli = 108

The % score of E. coli transformation efficiency = 106/108x100 = 1%

(Since there are more than 1 question, the first question have been answered according to the rules of Chegg)

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