DNA LAB PROCEDURE: 1. Record all information and data collected in your laborato

Question

DNA LAB

PROCEDURE:

1.    Record all information and data collected in your laboratory notebook, which is simply a spiral notebook or a piece of notebook paper.  

2.    Chill the rubbing alcohol by putting it on ice or in the refrigerator.

3.    Using the knife and cutting board, cut your DNA source into small pieces.

4.    Transfer DNA source to a small wide-mouth glass.

5.    Using a fork or mortar and pestle, mash the pieces of DNA source against the sides of the glass until you have a liquid mush. (You may use a blender to mash the DNA source and blend together the shampoo, salt and water with the DNA. Blending must be very brief (three pushes of the button) because blending can rapidly break the DNA into shorter lengths.)

6.    Add equal amount of water, several squirts of shampoo and about

Explanation / Answer

1. Helps to break open the cell.

2. Help to dissolve the cell membrane

3. To discombobulate lipid layers
4. Destroys the proteins that hold the DNA tightly coiled inside the cell
5. To force the DNA to precipitate out of solution
6. Deoxyribonucleic acid
7. 4
8. deoxyribose and sulfate group
9. Uracil
10. Guanine

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