1. Select three reasons your beta-carotene would not be the first pigment to elu

Question

1. Select three reasons your beta-carotene would not be the first pigment to elute off the column (Only select one of the following answers)

-the sample was not concentrated after extraction, the temp during elution was too high, or someone forgot to place a cotton plug in the bottom of the column

-the spinach leaves were not ground fine enough, the sodium sulfate drying step was skipped, or the sample was concentrated too much after extraction

-you used to incorrect adsorbent during TLC, the elutant was incorrect, or during extraction you discarded the wrong layer

-The elutent was incorrect, the sample was not concentrated after extraction, or sand was not used in the column during column chromatography

2. What two factors would effect how fast the beta-carotene moves in the chromatographic column

-how well the column is packed

-the polarity of the eluent

-The molar absorbtivity of the sample

-the color of the sample

-the temp of the eluent

Explanation / Answer

1.1,2,4

2.1,2

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