Suppose you can use siRNA to knockdown cytochrome C expression from cultured HeL

Question

Suppose you can use siRNA to knockdown cytochrome C expression from cultured HeLa cells, and furthermore that these cells can survive just fine using glycolysis as their sole source of ATP production. To test the relationship between cytochrome C and various apoptotic pathways, you will apply the following treatments in cyto C-depleted cells and then measure apoptosis. Based upon what we learned in class, predict the experimental outcomes and briefly rationalize each prediction. (1) Stimulate apoptosis using UV light-induced DNA damage (2) Stimulate apoptosis using FAS ligand added to culture media. (3) Stimulate apoptosis by overexpression of BAX. (4) Stimulate apoptosis by any of the methods above in the presence of the drug zVAD, a general caspase inhibitor. Suppose you can use siRNA to knockdown cytochrome C expression from cultured HeLa cells, and furthermore that these cells can survive just fine using glycolysis as their sole source of ATP production. To test the relationship between cytochrome C and various apoptotic pathways, you will apply the following treatments in cyto C-depleted cells and then measure apoptosis. Based upon what we learned in class, predict the experimental outcomes and briefly rationalize each prediction. (1) Stimulate apoptosis using UV light-induced DNA damage (2) Stimulate apoptosis using FAS ligand added to culture media. (3) Stimulate apoptosis by overexpression of BAX. (4) Stimulate apoptosis by any of the methods above in the presence of the drug zVAD, a general caspase inhibitor. Suppose you can use siRNA to knockdown cytochrome C expression from cultured HeLa cells, and furthermore that these cells can survive just fine using glycolysis as their sole source of ATP production. To test the relationship between cytochrome C and various apoptotic pathways, you will apply the following treatments in cyto C-depleted cells and then measure apoptosis. Based upon what we learned in class, predict the experimental outcomes and briefly rationalize each prediction. (1) Stimulate apoptosis using UV light-induced DNA damage (2) Stimulate apoptosis using FAS ligand added to culture media. (3) Stimulate apoptosis by overexpression of BAX. (4) Stimulate apoptosis by any of the methods above in the presence of the drug zVAD, a general caspase inhibitor.

Explanation / Answer

Apoptosis Ior programmed cell deathcan be initiated by two pathways-Intrinsic Pathway-Initiated through the release of signal factors by mitochondria within the cell.Generated inside the cell by the cellular stresses such as decreased oxygen.

Exrtrinsic pathway- Generated outside the cell.activated upon the binding of a death ligand to a death receptor on the cell membrane.receptors binds to the signalling molecules result cascade of caspases is activated which leads to the cell death.

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