The protein you identified in question 1 is important in regulation of chromatin
ID: 79446 • Letter: T
Question
The protein you identified in question 1 is important in regulation of chromatin structure (review what you know about it from the chromatin regulation lecture/reading). What assay could you use to determine all of the locations in the genome where this protein binds? What could you use as a positive control for the assay you described in part a? If you wanted to identify all of the proteins in the genome that could bind to the protein you identified in question 1d, what assay could you use? What protein could you use as a positive control for the assay you described in part c?Explanation / Answer
A) CHIP-SEQ
DNA sequencing to identify the genome wide binding sites of DNA-associated proteins.
B) For chip we need to isolate chromatin followed by Immunoprecipiation using beads against the protein in which you cloned your gene. so here we can use chromatin as a input and positive control.
C) LC-MS/MS after performing immunoprecipitation.
D) cell lysate can be used as input
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