In a non-denaturing protein electrophoresis (native protein electrophoresis), th

Question

In a non-denaturing protein electrophoresis (native protein electrophoresis), the movement of protein in acrylamide gel depends on Size only Size, charge and color Size, shape and net charge Size and shape Molecular biology techniques made it possible for Evolutionary Biologist to compare not only the physical structure of different organisms, but their Protein and DNA molecules True False Competitive inhibitors are molecules Bind to the product at the enzyme active site Binds to the enzyme at the substrate-enzyme active site Binds to the other site of enzyme and reduce enzyme activity All of the above a and c Suppose that a restriction enzyme has a recognition as 5'C G C'G'3'. We treated the following DNA sample (DNA sequence is shown below) with this restriction enzyme. Indicate: the number of the DNA fragments, b. the size of each fragment(Number of nucleotides)and c. write the base sequence of each DNA fragment Arrow indicates the cutting site 5' CA AGGG TG A T C T C G C G A AT T C G C G T A G T A A C G T T C CGT TCCAA T3'

Explanation / Answer

1. Number of DNA fragments: 3.

since there are 2 restriction sites for the enzyme so it will cut at to places and 3 fragmen ts wil be avilable

2. Fragment size: 15 nucleitdes, 8 nucleotides,21 nucleotides

3. Base sequence

first fragment:CA AGGG TGA TC TC GC

Second Fragment: G AA TT CGC

Third fragment : GTA GTA ACG TTC CGT TCCAAT

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