In a nondividing cell such as a liver cell, why must the flow of membrane betwee
ID: 7424 • Letter: I
Question
In a nondividing cell such as a liver cell, why must the flow of membrane between compartments be balanced, so that the retrieval pathways match the outward flow? Would you expect the same balanced flow in a gut epithelial cell, which is actively dividing?. Clathrin-coated vesicles bud from eukaryotic plasma membrane fragments when adaptins, clathrin, and dynamin-GTP are added. What would you expect to observe if the following modifications were made to the experiment? Explain your answer.
A. Adaptins were omitted.
B. Clathrin was omitted.
C. Dynamin was omitted.
D. Prokaryotic membrane fragments were used.
Explanation / Answer
Retrieval pathways match the outward flow to bring back the membrane and selected proteins back to the compartment of origin. The same balanced flow occurs in a gut epithelial cell, which is actively dividing, to facilitate the recycling of endosomes in transcytosis. A. Adaptins were omitted- assembling of clathrin coats is disturbed. B. Clathrin was omitted - Vescicle formation is altered as the clathrin coat assists in the bud formation by providing energy. C. Dynamin was omitted - It plays role in pinching off of clathrin coated vescicles. when it is omitted, it either enhance or inhibit the pinching off process. D. Prokaryotic membrane fragments were used - These processes are specific to eucaryotes.
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