1. Calculate the amount of 0.1 M sucrose solution and the amount of dH2O needed

Question

1. Calculate the amount of 0.1 M sucrose solution and the amount of dH2O needed to prepare 100 mL of unbuffered 20 mM sucrose.[Hint: use the formula c1v1 = c2v2 to calculate the amount of sucrose stock solution needed.]
Amount of 0.1 M sucrose solution needed: Amount of  dH2O needed:
2. A chemistry reference indicates that a solution containing 40 mM acetic acid and 60 mM sodium acetate (a source of acetate ions) will produce an acetate buffer with a pH of 4.8. Calculate the amount of each stock solution and the amount of dH2O needed to prepare 100 mL of an acetate buffer that contains 20 mM sucrose, 40 mM acetic acid, and 60 mM sodium acetate. [Hint: use the formula c1v1= c2v2 to calculate the amount of each stock solution needed.]
Amount of 0.1 M sucrose solution needed: Amount of 0.2 M acetic acid solution needed: Amount of 0.2 M sodium acetate solution needed: Amount of dH2O needed: 1. Calculate the amount of 0.1 M sucrose solution and the amount of dH2O needed to prepare 100 mL of unbuffered 20 mM sucrose.[Hint: use the formula c1v1 = c2v2 to calculate the amount of sucrose stock solution needed.]
Amount of 0.1 M sucrose solution needed: Amount of  dH2O needed:
2. A chemistry reference indicates that a solution containing 40 mM acetic acid and 60 mM sodium acetate (a source of acetate ions) will produce an acetate buffer with a pH of 4.8. Calculate the amount of each stock solution and the amount of dH2O needed to prepare 100 mL of an acetate buffer that contains 20 mM sucrose, 40 mM acetic acid, and 60 mM sodium acetate. [Hint: use the formula c1v1= c2v2 to calculate the amount of each stock solution needed.]
Amount of 0.1 M sucrose solution needed: Amount of 0.2 M acetic acid solution needed: Amount of 0.2 M sodium acetate solution needed: Amount of dH2O needed: Amount of 0.1 M sucrose solution needed: Amount of 0.2 M acetic acid solution needed: Amount of 0.2 M sodium acetate solution needed: Amount of dH2O needed:

Explanation / Answer

2. A chemistry reference indicates that a solution containing 40 mM acetic acid and 60 mM sodium acetate (a source of acetate ions) will produce an acetate buffer with a pH of 4.8. Calculate the amount of each stock solution and the amount of dH2O needed to prepare 100 mL of an acetate buffer that contains 20 mM sucrose, 40 mM acetic acid, and 60 mM sodium acetate. [Hint: use the formula c1v1= c2v2 to calculate the amount of each stock solution needed.]
Amount of 0.1 M sucrose solution needed:
ANSWER =20 mL Amount of 0.2 M acetic acid solution needed: ANSWER =20 mL
Amount of 0.2 M sodium acetate solution needed:
ANSWER =30 mL Amount of dH2O needed: ANSWER =30 mL Amount of 0.1 M sucrose solution needed:
ANSWER =20 mL Amount of 0.2 M acetic acid solution needed: ANSWER =20 mL
Amount of 0.2 M sodium acetate solution needed:
ANSWER =30 mL Amount of dH2O needed: ANSWER =30 mL

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